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gibco,DMEM高糖培养基

  • 产品型号:11995-065
  • 产品时间:2016/05/18 12:11:43
  • 简要描述:gibco,DMEM高糖培养基 11995-065 DMEM大量到货
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  • 产品简介

DMEM, high glucose, pyruvate

Description

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the  selector tool.

This DMEM is modified as follows:
With Without
• High Glucose • HEPES
• L-glutamine  
• Phenol Red  
• Sodium Pyruvate  


The complete formulation is available.

Using DMEM
DMEM is unique from other as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Product use
For human ex vivo tissue and cell culture processing applications. CAUTION: When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco® DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).
 

Specifications

 

Glutamine: L-Glutamine
Phenol Red Indicator: Phenol Red
Form: Liquid
Glucose: High Glucose
Serum Supplementation: Standard Serum Supplementation
HEPES Buffer: No HEPES
Sodium Pyruvate Additive: Sodium Pyruvate
Product Size: 10 x 500 mL
Volume (Metric): 500 ml
Shipping Condition: Room Temperature

 

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